Crosstalk between CRISPR-Cas9 and the human transcriptome

CRISPR-Cas9 expression independent of its cognate synthetic guide RNA (gRNA) causes widespread genomic DNA damage in human cells. To investigate whether Cas9 can interact with endogenous human RNA transcripts independent of its guide, we perform eCLIP (enhanced CLIP) of Cas9 in human cells and find...

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Detalles Bibliográficos
Autores principales: Smargon, Aaron A., Madrigal, Assael A., Yee, Brian A., Dong, Kevin D., Mueller, Jasmine R., Yeo, Gene W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891275/
https://www.ncbi.nlm.nih.gov/pubmed/35236841
http://dx.doi.org/10.1038/s41467-022-28719-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891275/
https://www.ncbi.nlm.nih.gov/pubmed/35236841
http://dx.doi.org/10.1038/s41467-022-28719-5

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