Cargando…

Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader

Sliding clamps are ring-shaped protein complexes that are integral to the DNA replication machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader AAA+ ATPase complexes. However, how a clamp loader opens and closes the sliding clamp around DNA is still unknown. Here, w...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gaubitz, Christl, Liu, Xingchen, Pajak, Joshua, Stone, Nicholas P, Hayes, Janelle A, Demo, Gabriel, Kelch, Brian A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2022
Materias:	Structural Biology and Molecular Biophysics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8893722/ https://www.ncbi.nlm.nih.gov/pubmed/35179493 http://dx.doi.org/10.7554/eLife.74175

Ejemplares similares

A second DNA binding site on RFC facilitates clamp loading at gapped or nicked DNA
por: Liu, Xingchen, et al.
Publicado: (2022)

Allosteric communication in DNA polymerase clamp loaders relies on a critical hydrogen-bonded junction
por: Subramanian, Subu, et al.
Publicado: (2021)

cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ
por: Fernandez-Leiro, Rafael, et al.
Publicado: (2015)

New tools for automated high-resolution cryo-EM structure determination in RELION-3
por: Zivanov, Jasenko, et al.
Publicado: (2018)

Cryo-EM structure of respiratory complex I at work
por: Parey, Kristian, et al.
Publicado: (2018)

Multistep loading of a DNA sliding clamp onto DNA by replication factor C
por: Schrecker, Marina, et al.
Publicado: (2022)

Model-based local density sharpening of cryo-EM maps
por: Jakobi, Arjen J, et al.
Publicado: (2017)

Cryo-EM structure of the bifunctional secretin complex of Thermus thermophilus
por: D'Imprima, Edoardo, et al.
Publicado: (2017)

Cryo-EM structure of VASH1-SVBP bound to microtubules
por: Li, Faxiang, et al.
Publicado: (2020)

Automated systematic evaluation of cryo-EM specimens with SmartScope
por: Bouvette, Jonathan, et al.
Publicado: (2022)

Cryo-EM structure of the polycystin 2-l1 ion channel
por: Hulse, Raymond E, et al.
Publicado: (2018)

Routine single particle CryoEM sample and grid characterization by tomography
por: Noble, Alex J, et al.
Publicado: (2018)

Cryo-EM analysis of PIP(2) regulation in mammalian GIRK channels
por: Niu, Yiming, et al.
Publicado: (2020)

Cryo-EM structures and functional properties of CALHM channels of the human placenta
por: Drożdżyk, Katarzyna, et al.
Publicado: (2020)

Cryo-EM enters a new era
por: Kühlbrandt, Werner
Publicado: (2014)

All-atom molecular dynamics of the HBV capsid reveals insights into biological function and cryo-EM resolution limits
por: Hadden, Jodi A, et al.
Publicado: (2018)

Automated cryo-EM structure refinement using correlation-driven molecular dynamics
por: Igaev, Maxim, et al.
Publicado: (2019)

Mechanism of pharmacochaperoning in a mammalian K(ATP) channel revealed by cryo-EM
por: Martin, Gregory M, et al.
Publicado: (2019)

Cryo-EM structure of the mechanically activated ion channel OSCA1.2
por: Jojoa-Cruz, Sebastian, et al.
Publicado: (2018)

The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1
por: Sosnowski, Piotr, et al.
Publicado: (2018)

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
por: Wang, Ling, et al.
Publicado: (2020)

Cryo-EM structure of the potassium-chloride cotransporter KCC4 in lipid nanodiscs
por: Reid, Michelle S, et al.
Publicado: (2020)

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes
por: Huber, Stefan T, et al.
Publicado: (2022)

Cryo-EM structures of mitochondrial respiratory complex I from Drosophila melanogaster
por: Agip, Ahmed-Noor A, et al.
Publicado: (2023)

Cryo-EM structure of the endothelin-1-ET(B)-G(i) complex
por: Sano, Fumiya K, et al.
Publicado: (2023)

High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms
por: Arragain, Benoît, et al.
Publicado: (2019)

Cryo-EM structure of the SAGA and NuA4 coactivator subunit Tra1 at 3.7 angstrom resolution
por: Díaz-Santín, Luis Miguel, et al.
Publicado: (2017)

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles
por: Bai, Xiao-chen, et al.
Publicado: (2013)

Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F
por: Alvadia, Carolina, et al.
Publicado: (2019)

Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations
por: Xue, Jing, et al.
Publicado: (2020)

Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands
por: Li, Jie, et al.
Publicado: (2019)

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme
por: Zhang, Zhening, et al.
Publicado: (2018)

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION
por: Nakane, Takanori, et al.
Publicado: (2018)

Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule
por: Lacey, Samuel E, et al.
Publicado: (2019)

Cryo-EM structure of the lysosomal chloride-proton exchanger CLC-7 in complex with OSTM1
por: Schrecker, Marina, et al.
Publicado: (2020)

The cryo-EM structure of the human uromodulin filament core reveals a unique assembly mechanism
por: Stanisich, Jessica J, et al.
Publicado: (2020)

Cryo-EM structures demonstrate human IMPDH2 filament assembly tunes allosteric regulation
por: Johnson, Matthew C, et al.
Publicado: (2020)

Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2
por: Xie, Yihu, et al.
Publicado: (2021)

Cryo-EM structures of the caspase-activated protein XKR9 involved in apoptotic lipid scrambling
por: Straub, Monique S, et al.
Publicado: (2021)

Cryo-EM structures of an LRRC8 chimera with native functional properties reveal heptameric assembly
por: Takahashi, Hirohide, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_o71q5f3u6oedktj03t7u9g6i21