Long-read sequencing for identification of insertion sites in large transposon mutant libraries

Transposon insertion site sequencing (TIS) is a powerful method for associating genotype to phenotype. However, all TIS methods described to date use short nucleotide sequence reads which cannot uniquely determine the locations of transposon insertions within repeating genomic sequences where the re...

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Detalles Bibliográficos
Autores principales: Yasir, Muhammad, Turner, A. Keith, Lott, Martin, Rudder, Steven, Baker, David, Bastkowski, Sarah, Page, Andrew J., Webber, Mark A., Charles, Ian G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894413/
https://www.ncbi.nlm.nih.gov/pubmed/35241765
http://dx.doi.org/10.1038/s41598-022-07557-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894413/
https://www.ncbi.nlm.nih.gov/pubmed/35241765
http://dx.doi.org/10.1038/s41598-022-07557-x

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