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Long-read sequencing for identification of insertion sites in large transposon mutant libraries
Transposon insertion site sequencing (TIS) is a powerful method for associating genotype to phenotype. However, all TIS methods described to date use short nucleotide sequence reads which cannot uniquely determine the locations of transposon insertions within repeating genomic sequences where the re...
Autores principales: | Yasir, Muhammad, Turner, A. Keith, Lott, Martin, Rudder, Steven, Baker, David, Bastkowski, Sarah, Page, Andrew J., Webber, Mark A., Charles, Ian G. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894413/ https://www.ncbi.nlm.nih.gov/pubmed/35241765 http://dx.doi.org/10.1038/s41598-022-07557-x |
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