Low-input RNase footprinting for simultaneous quantification of cytosolic and mitochondrial translation

We describe a low-input RNase footprinting approach for the rapid quantification of ribosome-protected fragments with as few as 1000 cultured cells. The assay uses a simplified procedure to selectively capture ribosome footprints based on optimized RNase digestion. It simultaneously maps cytosolic a...

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Detalles Bibliográficos
Autores principales: Li, Qianru, Yang, Haiwang, Stroup, Emily K., Wang, Hongbin, Ji, Zhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896460/
https://www.ncbi.nlm.nih.gov/pubmed/35193938
http://dx.doi.org/10.1101/gr.276139.121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896460/
https://www.ncbi.nlm.nih.gov/pubmed/35193938
http://dx.doi.org/10.1101/gr.276139.121

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