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A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we pro...

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Detalles Bibliográficos
Autores principales: Binó, Lucia, Mikulenková, Erika, Štepánek, Luděk, Bernatík, Ondřej, Vysloužil, David, Pejšková, Petra, Gorilák, Peter, Huranová, Martina, Varga, Vladimír, Čajánek, Lukáš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897589/
https://www.ncbi.nlm.nih.gov/pubmed/35257113
http://dx.doi.org/10.1016/j.xpro.2022.101199