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A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we pro...

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Detalles Bibliográficos
Autores principales: Binó, Lucia, Mikulenková, Erika, Štepánek, Luděk, Bernatík, Ondřej, Vysloužil, David, Pejšková, Petra, Gorilák, Peter, Huranová, Martina, Varga, Vladimír, Čajánek, Lukáš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897589/
https://www.ncbi.nlm.nih.gov/pubmed/35257113
http://dx.doi.org/10.1016/j.xpro.2022.101199
Descripción
Sumario:Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).