A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we pro...

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Autores principales: Binó, Lucia, Mikulenková, Erika, Štepánek, Luděk, Bernatík, Ondřej, Vysloužil, David, Pejšková, Petra, Gorilák, Peter, Huranová, Martina, Varga, Vladimír, Čajánek, Lukáš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897589/
https://www.ncbi.nlm.nih.gov/pubmed/35257113
http://dx.doi.org/10.1016/j.xpro.2022.101199
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author Binó, Lucia
Mikulenková, Erika
Štepánek, Luděk
Bernatík, Ondřej
Vysloužil, David
Pejšková, Petra
Gorilák, Peter
Huranová, Martina
Varga, Vladimír
Čajánek, Lukáš
author_facet Binó, Lucia
Mikulenková, Erika
Štepánek, Luděk
Bernatík, Ondřej
Vysloužil, David
Pejšková, Petra
Gorilák, Peter
Huranová, Martina
Varga, Vladimír
Čajánek, Lukáš
author_sort Binó, Lucia
collection PubMed
description Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
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spelling pubmed-88975892022-03-06 A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines Binó, Lucia Mikulenková, Erika Štepánek, Luděk Bernatík, Ondřej Vysloužil, David Pejšková, Petra Gorilák, Peter Huranová, Martina Varga, Vladimír Čajánek, Lukáš STAR Protoc Protocol Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020). Elsevier 2022-03-02 /pmc/articles/PMC8897589/ /pubmed/35257113 http://dx.doi.org/10.1016/j.xpro.2022.101199 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Binó, Lucia
Mikulenková, Erika
Štepánek, Luděk
Bernatík, Ondřej
Vysloužil, David
Pejšková, Petra
Gorilák, Peter
Huranová, Martina
Varga, Vladimír
Čajánek, Lukáš
A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
title A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
title_full A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
title_fullStr A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
title_full_unstemmed A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
title_short A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
title_sort protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897589/
https://www.ncbi.nlm.nih.gov/pubmed/35257113
http://dx.doi.org/10.1016/j.xpro.2022.101199
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