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A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we pro...
Autores principales: | Binó, Lucia, Mikulenková, Erika, Štepánek, Luděk, Bernatík, Ondřej, Vysloužil, David, Pejšková, Petra, Gorilák, Peter, Huranová, Martina, Varga, Vladimír, Čajánek, Lukáš |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897589/ https://www.ncbi.nlm.nih.gov/pubmed/35257113 http://dx.doi.org/10.1016/j.xpro.2022.101199 |
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