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Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity

Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC...

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Detalles Bibliográficos
Autores principales: Bergin, Christopher J., Benoit, Yannick D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899043/
https://www.ncbi.nlm.nih.gov/pubmed/35265864
http://dx.doi.org/10.1016/j.xpro.2022.101218