Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity

Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC...

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Detalles Bibliográficos
Autores principales: Bergin, Christopher J., Benoit, Yannick D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899043/
https://www.ncbi.nlm.nih.gov/pubmed/35265864
http://dx.doi.org/10.1016/j.xpro.2022.101218
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author Bergin, Christopher J.
Benoit, Yannick D.
author_facet Bergin, Christopher J.
Benoit, Yannick D.
author_sort Bergin, Christopher J.
collection PubMed
description Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques. For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021).
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spelling pubmed-88990432022-03-08 Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity Bergin, Christopher J. Benoit, Yannick D. STAR Protoc Protocol Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques. For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021). Elsevier 2022-03-04 /pmc/articles/PMC8899043/ /pubmed/35265864 http://dx.doi.org/10.1016/j.xpro.2022.101218 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bergin, Christopher J.
Benoit, Yannick D.
Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
title Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
title_full Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
title_fullStr Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
title_full_unstemmed Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
title_short Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
title_sort protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899043/
https://www.ncbi.nlm.nih.gov/pubmed/35265864
http://dx.doi.org/10.1016/j.xpro.2022.101218
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