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Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899043/ https://www.ncbi.nlm.nih.gov/pubmed/35265864 http://dx.doi.org/10.1016/j.xpro.2022.101218 |