Analysis of the pheromone signaling pathway by RT-qPCR in the budding yeast Saccharomyces cerevisiae

FUS3 and STE2 expression levels can be used as reporters for signaling through the pheromone pathway in the budding yeast Saccharomyces cerevisiae. Here, we describe an optimized protocol to measure the expression levels of FUS3 and STE2 using quantitative reverse transcription PCR (RT-qPCR). We des...

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Detalles Bibliográficos
Autores principales: Ramos-Alonso, Lucía, Garcia, Ignacio, Enserink, Jorrit M., Chymkowitch, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899044/
https://www.ncbi.nlm.nih.gov/pubmed/35265859
http://dx.doi.org/10.1016/j.xpro.2022.101210

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899044/
https://www.ncbi.nlm.nih.gov/pubmed/35265859
http://dx.doi.org/10.1016/j.xpro.2022.101210

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