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Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo
Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development in vivo. Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis in vivo using hydrodynamic injection of plasmids encoding cytokines...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899045/ https://www.ncbi.nlm.nih.gov/pubmed/35265863 http://dx.doi.org/10.1016/j.xpro.2022.101217 |
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author | Ren, Deshan Liu, Wei Ding, Shuai Li, Yan |
author_facet | Ren, Deshan Liu, Wei Ding, Shuai Li, Yan |
author_sort | Ren, Deshan |
collection | PubMed |
description | Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development in vivo. Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis in vivo using hydrodynamic injection of plasmids encoding cytokines of interest, and flow cytometry analysis of humanization levels and human immune subsets. This approach can be easily applied to screen or verify factors that regulate human hematopoiesis and immune system. For complete details on the use and execution of this protocol, please refer to Cardoso et al. (2021) and Li et al. (2017). |
format | Online Article Text |
id | pubmed-8899045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-88990452022-03-08 Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo Ren, Deshan Liu, Wei Ding, Shuai Li, Yan STAR Protoc Protocol Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development in vivo. Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis in vivo using hydrodynamic injection of plasmids encoding cytokines of interest, and flow cytometry analysis of humanization levels and human immune subsets. This approach can be easily applied to screen or verify factors that regulate human hematopoiesis and immune system. For complete details on the use and execution of this protocol, please refer to Cardoso et al. (2021) and Li et al. (2017). Elsevier 2022-03-03 /pmc/articles/PMC8899045/ /pubmed/35265863 http://dx.doi.org/10.1016/j.xpro.2022.101217 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Ren, Deshan Liu, Wei Ding, Shuai Li, Yan Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
title | Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
title_full | Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
title_fullStr | Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
title_full_unstemmed | Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
title_short | Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
title_sort | protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899045/ https://www.ncbi.nlm.nih.gov/pubmed/35265863 http://dx.doi.org/10.1016/j.xpro.2022.101217 |
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