Development of Fluorescence Polarization Immunoassay With scFv to Detect Fumonisin B(s) in Maize and Simultaneous Study of Their Molecular Recognition Mechanism

In this study, a fluorescence polarization immunoassay (FPIA) was developed based on the single-chain variable fragments (scFvs) for fumonisin B(s) (FB(s)). The scFvs were prepared from FB(s)-specific monoclonal antibody secreting hybridomas (4F5 and 4B9). The established FPIA could determine the su...

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Detalles Bibliográficos
Autores principales: Li, Yuan, Yu, Qing, Yu, Wenbo, Zhang, Suxia, Wen, Kai, Shen, Jianzhong, Wang, Zhanhui, Yu, Xuezhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900220/
https://www.ncbi.nlm.nih.gov/pubmed/35265585
http://dx.doi.org/10.3389/fchem.2022.829038

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900220/
https://www.ncbi.nlm.nih.gov/pubmed/35265585
http://dx.doi.org/10.3389/fchem.2022.829038

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