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Development of Fluorescence Polarization Immunoassay With scFv to Detect Fumonisin B(s) in Maize and Simultaneous Study of Their Molecular Recognition Mechanism
In this study, a fluorescence polarization immunoassay (FPIA) was developed based on the single-chain variable fragments (scFvs) for fumonisin B(s) (FB(s)). The scFvs were prepared from FB(s)-specific monoclonal antibody secreting hybridomas (4F5 and 4B9). The established FPIA could determine the su...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900220/ https://www.ncbi.nlm.nih.gov/pubmed/35265585 http://dx.doi.org/10.3389/fchem.2022.829038 |