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A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation

BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segme...

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Detalles Bibliográficos
Autores principales: Mori, Kotaro, Fukui, Kaho, Amatsu, Ryotaro, Ishikawa, Shu, Verrone, Valeria, Wipat, Anil, Meijer, Wilfried J. J., Yoshida, Ken-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903633/
https://www.ncbi.nlm.nih.gov/pubmed/35260160
http://dx.doi.org/10.1186/s12934-022-01759-8