A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation

BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segme...

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Autores principales: Mori, Kotaro, Fukui, Kaho, Amatsu, Ryotaro, Ishikawa, Shu, Verrone, Valeria, Wipat, Anil, Meijer, Wilfried J. J., Yoshida, Ken-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903633/
https://www.ncbi.nlm.nih.gov/pubmed/35260160
http://dx.doi.org/10.1186/s12934-022-01759-8
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author Mori, Kotaro
Fukui, Kaho
Amatsu, Ryotaro
Ishikawa, Shu
Verrone, Valeria
Wipat, Anil
Meijer, Wilfried J. J.
Yoshida, Ken-ichi
author_facet Mori, Kotaro
Fukui, Kaho
Amatsu, Ryotaro
Ishikawa, Shu
Verrone, Valeria
Wipat, Anil
Meijer, Wilfried J. J.
Yoshida, Ken-ichi
author_sort Mori, Kotaro
collection PubMed
description BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis. RESULTS: In this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation. CONCLUSIONS: This transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-022-01759-8.
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spelling pubmed-89036332022-03-18 A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation Mori, Kotaro Fukui, Kaho Amatsu, Ryotaro Ishikawa, Shu Verrone, Valeria Wipat, Anil Meijer, Wilfried J. J. Yoshida, Ken-ichi Microb Cell Fact Research BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis. RESULTS: In this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation. CONCLUSIONS: This transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-022-01759-8. BioMed Central 2022-03-08 /pmc/articles/PMC8903633/ /pubmed/35260160 http://dx.doi.org/10.1186/s12934-022-01759-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mori, Kotaro
Fukui, Kaho
Amatsu, Ryotaro
Ishikawa, Shu
Verrone, Valeria
Wipat, Anil
Meijer, Wilfried J. J.
Yoshida, Ken-ichi
A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
title A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
title_full A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
title_fullStr A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
title_full_unstemmed A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
title_short A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
title_sort novel method for transforming geobacillus kaustophilus with a chromosomal segment of bacillus subtilis transferred via pls20-dependent conjugation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903633/
https://www.ncbi.nlm.nih.gov/pubmed/35260160
http://dx.doi.org/10.1186/s12934-022-01759-8
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