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A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segme...
Autores principales: | Mori, Kotaro, Fukui, Kaho, Amatsu, Ryotaro, Ishikawa, Shu, Verrone, Valeria, Wipat, Anil, Meijer, Wilfried J. J., Yoshida, Ken-ichi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903633/ https://www.ncbi.nlm.nih.gov/pubmed/35260160 http://dx.doi.org/10.1186/s12934-022-01759-8 |
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