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Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach
Inosine monophosphate (IMP) is an indicator of meat taste, and the molecular mechanism underlying IMP deposition in muscle tissues is important to developing superior poultry breeds. The aim of this study was to identify the key proteins regulating IMP deposition in different muscle groups of 180-da...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904228/ https://www.ncbi.nlm.nih.gov/pubmed/35259688 http://dx.doi.org/10.1016/j.psj.2022.101741 |
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author | Huang, Zengwen Zhang, Juan Gu, Yaling Cai, Zhengyun Wei, Dawei Feng, Xiaofang Yang, Chaoyun |
author_facet | Huang, Zengwen Zhang, Juan Gu, Yaling Cai, Zhengyun Wei, Dawei Feng, Xiaofang Yang, Chaoyun |
author_sort | Huang, Zengwen |
collection | PubMed |
description | Inosine monophosphate (IMP) is an indicator of meat taste, and the molecular mechanism underlying IMP deposition in muscle tissues is important to developing superior poultry breeds. The aim of this study was to identify the key proteins regulating IMP deposition in different muscle groups of 180-day-old Jingyuan chickens (Hen) using a proteomics-based approach. We identified 1,300 proteins in the muscle tissues of Jingyuan chickens, of which 322 were differentially expressed between the breast and leg muscles (129 proteins were highly expressed in breast muscles and 193 proteins were highly expressed in leg muscles). PGM1, PKM2, AK1, AMPD1, and PurH/ATIC were among the differentially expressed proteins (DEPs) involved in the purine metabolism pathway, of which purH was highly expressed in leg muscles, while the others were highly expressed in breast muscles. The proteomics screening results were verified by PRM, qPCR, and western blotting, showing consistency with the proteomics results. Our findings are not only significant in terms of protecting the Jingyuan chicken germplasm resources, but also provide the molecular basis for generating high-quality broiler chicken breeds. |
format | Online Article Text |
id | pubmed-8904228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89042282022-03-10 Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach Huang, Zengwen Zhang, Juan Gu, Yaling Cai, Zhengyun Wei, Dawei Feng, Xiaofang Yang, Chaoyun Poult Sci METABOLISM AND NUTRITION Inosine monophosphate (IMP) is an indicator of meat taste, and the molecular mechanism underlying IMP deposition in muscle tissues is important to developing superior poultry breeds. The aim of this study was to identify the key proteins regulating IMP deposition in different muscle groups of 180-day-old Jingyuan chickens (Hen) using a proteomics-based approach. We identified 1,300 proteins in the muscle tissues of Jingyuan chickens, of which 322 were differentially expressed between the breast and leg muscles (129 proteins were highly expressed in breast muscles and 193 proteins were highly expressed in leg muscles). PGM1, PKM2, AK1, AMPD1, and PurH/ATIC were among the differentially expressed proteins (DEPs) involved in the purine metabolism pathway, of which purH was highly expressed in leg muscles, while the others were highly expressed in breast muscles. The proteomics screening results were verified by PRM, qPCR, and western blotting, showing consistency with the proteomics results. Our findings are not only significant in terms of protecting the Jingyuan chicken germplasm resources, but also provide the molecular basis for generating high-quality broiler chicken breeds. Elsevier 2022-01-30 /pmc/articles/PMC8904228/ /pubmed/35259688 http://dx.doi.org/10.1016/j.psj.2022.101741 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | METABOLISM AND NUTRITION Huang, Zengwen Zhang, Juan Gu, Yaling Cai, Zhengyun Wei, Dawei Feng, Xiaofang Yang, Chaoyun Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach |
title | Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach |
title_full | Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach |
title_fullStr | Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach |
title_full_unstemmed | Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach |
title_short | Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach |
title_sort | analysis of the molecular mechanism of inosine monophosphate deposition in jingyuan chicken muscles using a proteomic approach |
topic | METABOLISM AND NUTRITION |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904228/ https://www.ncbi.nlm.nih.gov/pubmed/35259688 http://dx.doi.org/10.1016/j.psj.2022.101741 |
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