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In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resoluti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904606/ https://www.ncbi.nlm.nih.gov/pubmed/35284836 http://dx.doi.org/10.1016/j.xpro.2022.101224 |