In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resoluti...

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Detalles Bibliográficos
Autores principales: Gershon, Devora, Negev-Goldstein, Robert H., Abd al Razzaq, Lama, Lev, Shaya, Binshtok, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904606/
https://www.ncbi.nlm.nih.gov/pubmed/35284836
http://dx.doi.org/10.1016/j.xpro.2022.101224
Descripción
Sumario:This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

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