In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resoluti...

Descripción completa

Detalles Bibliográficos
Autores principales: Gershon, Devora, Negev-Goldstein, Robert H., Abd al Razzaq, Lama, Lev, Shaya, Binshtok, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904606/
https://www.ncbi.nlm.nih.gov/pubmed/35284836
http://dx.doi.org/10.1016/j.xpro.2022.101224
_version_ 1784664991525240832
author Gershon, Devora
Negev-Goldstein, Robert H.
Abd al Razzaq, Lama
Lev, Shaya
Binshtok, Alexander M.
author_facet Gershon, Devora
Negev-Goldstein, Robert H.
Abd al Razzaq, Lama
Lev, Shaya
Binshtok, Alexander M.
author_sort Gershon, Devora
collection PubMed
description This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).
format Online
Article
Text
id pubmed-8904606
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-89046062022-03-10 In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals Gershon, Devora Negev-Goldstein, Robert H. Abd al Razzaq, Lama Lev, Shaya Binshtok, Alexander M. STAR Protoc Protocol This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019). Elsevier 2022-03-04 /pmc/articles/PMC8904606/ /pubmed/35284836 http://dx.doi.org/10.1016/j.xpro.2022.101224 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gershon, Devora
Negev-Goldstein, Robert H.
Abd al Razzaq, Lama
Lev, Shaya
Binshtok, Alexander M.
In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
title In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
title_full In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
title_fullStr In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
title_full_unstemmed In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
title_short In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
title_sort in vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904606/
https://www.ncbi.nlm.nih.gov/pubmed/35284836
http://dx.doi.org/10.1016/j.xpro.2022.101224
work_keys_str_mv AT gershondevora invivoopticalrecordingsofiondynamicsinmousecornealprimarynociceptiveterminals
AT negevgoldsteinroberth invivoopticalrecordingsofiondynamicsinmousecornealprimarynociceptiveterminals
AT abdalrazzaqlama invivoopticalrecordingsofiondynamicsinmousecornealprimarynociceptiveterminals
AT levshaya invivoopticalrecordingsofiondynamicsinmousecornealprimarynociceptiveterminals
AT binshtokalexanderm invivoopticalrecordingsofiondynamicsinmousecornealprimarynociceptiveterminals

Ejemplares similares