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In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resoluti...

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Detalles Bibliográficos
Autores principales: Gershon, Devora, Negev-Goldstein, Robert H., Abd al Razzaq, Lama, Lev, Shaya, Binshtok, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904606/
https://www.ncbi.nlm.nih.gov/pubmed/35284836
http://dx.doi.org/10.1016/j.xpro.2022.101224