A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system

Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) single-cell isolation; and (4) knockout verificatio...

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Detalles Bibliográficos
Autores principales: Aouida, Mustapha, Aljogol, Dina, Ali, Reem, Ramotar, Dindial
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904610/
https://www.ncbi.nlm.nih.gov/pubmed/35284843
http://dx.doi.org/10.1016/j.xpro.2022.101216

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904610/
https://www.ncbi.nlm.nih.gov/pubmed/35284843
http://dx.doi.org/10.1016/j.xpro.2022.101216

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