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A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system
Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) single-cell isolation; and (4) knockout verificatio...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904610/ https://www.ncbi.nlm.nih.gov/pubmed/35284843 http://dx.doi.org/10.1016/j.xpro.2022.101216 |