Toehold-mediated strand displacement to measure released product from self-cleaving ribozymes

This paper presents a probe comprising a fluorophore and a quencher, enabling measurement of released product from self-cleaving hammerhead ribozyme, without labeled RNA molecules, regular sampling or use of polyacrylamide gels. The probe is made of two DNA strands; one strand is labeled with a fluo...

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Detalles Bibliográficos
Autores principales: Kapadia, Jay Bhakti, Kharma, Nawwaf, Davis, Alen Nellikulam, Kamel, Nicolas, Perreault, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906547/
https://www.ncbi.nlm.nih.gov/pubmed/34862273
http://dx.doi.org/10.1261/rna.078823.121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906547/
https://www.ncbi.nlm.nih.gov/pubmed/34862273
http://dx.doi.org/10.1261/rna.078823.121

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