Cargando…

Structural basis for mismatch surveillance by CRISPR–Cas9

CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage(1–4), and the underlying mechanisms by which Cas9 recognizes mismatches are poorly understood(5–7). Although Cas9 variants with greater discrimination against mismatches have been designed(8–10), these suffer fr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bravo, Jack P. K., Liu, Mu-Sen, Hibshman, Grace N., Dangerfield, Tyler L., Jung, Kyungseok, McCool, Ryan S., Johnson, Kenneth A., Taylor, David W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907077/ https://www.ncbi.nlm.nih.gov/pubmed/35236982 http://dx.doi.org/10.1038/s41586-022-04470-1

Ejemplares similares

Publisher Correction: Structural basis for mismatch surveillance by CRISPR–Cas9
por: Bravo, Jack P. K., et al.
Publicado: (2022)

Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
por: Liu, Mu-Sen, et al.
Publicado: (2020)

Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9
por: Mitchell, Brandon P., et al.
Publicado: (2020)

Type III CRISPR-Cas complexes act as protein-assisted ribozymes during target RNA cleavage
por: Schwartz, Evan A., et al.
Publicado: (2023)

CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs
por: Lee, Ho Joung, et al.
Publicado: (2020)

Prediction of CRISPR-Cas9 off-target activities with mismatches and indels based on hybrid neural network
por: Yang, Yanpeng, et al.
Publicado: (2023)

R-CRISPR: A Deep Learning Network to Predict Off-Target Activities with Mismatch, Insertion and Deletion in CRISPR-Cas9 System
por: Niu, Rui, et al.
Publicado: (2021)

What is CRISPR/Cas9?
por: Redman, Melody, et al.
Publicado: (2016)

Advances in CRISPR/Cas9
por: Zhu, Youmin
Publicado: (2022)

The Advance of CRISPR-Cas9-Based and NIR/CRISPR-Cas9-Based Imaging System
por: Qiao, Huanhuan, et al.
Publicado: (2021)

Structural insights into Cas9 mismatch: promising for development of high-fidelity Cas9 variants
por: Tang, Honghai, et al.
Publicado: (2022)

Relationship between DNA mismatch repair and CRISPR/Cas9-mediated knock-in in the bovine β-casein gene locus
por: Kim, Seung-Yeon, et al.
Publicado: (2022)

Conformational dynamics during misincorporation and mismatch extension defined using a DNA polymerase with a fluorescent artificial amino acid
por: Dangerfield, Tyler L., et al.
Publicado: (2021)

Disarming of type I-F CRISPR-Cas surveillance complex by anti-CRISPR proteins AcrIF6 and AcrIF9
por: Kupcinskaite, Egle, et al.
Publicado: (2022)

CRISPR/Cas9 in Gastrointestinal Malignancies
por: Jefremow, André, et al.
Publicado: (2021)

Mismatch Intolerance of 5′-Truncated sgRNAs in CRISPR/Cas9 Enables Efficient Microbial Single-Base Genome Editing
por: Lee, Ho Joung, et al.
Publicado: (2021)

Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
por: Zhang, Song, et al.
Publicado: (2021)

Structure of a type IV CRISPR-Cas ribonucleoprotein complex
por: Zhou, Yi, et al.
Publicado: (2021)

Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo
por: Fu, Becky X.H., et al.
Publicado: (2016)

The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit
por: Bean, Björn D M, et al.
Publicado: (2022)

The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system
por: Nasef, Mohamed, et al.
Publicado: (2022)

An Agrobacterium‐delivered CRISPR/Cas9 system for targeted mutagenesis in sorghum
por: Char, Si Nian, et al.
Publicado: (2019)

Application of CRISPR/Cas9 System in Establishing Large Animal Models
por: Lin, Yingqi, et al.
Publicado: (2022)

Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication
por: Bravo, Jack P.K., et al.
Publicado: (2021)

Application of CRISPR/Cas9 Technology to HBV
por: Lin, Guigao, et al.
Publicado: (2015)

Chromosome engineering in zygotes with CRISPR/Cas9
por: Boroviak, Katharina, et al.
Publicado: (2016)

Application of CRISPR/Cas9 for biomedical discoveries
por: Riordan, Sean M., et al.
Publicado: (2015)

Application of CRISPR/Cas9 in plant biology
por: Liu, Xuan, et al.
Publicado: (2017)

Reshuffling yeast chromosomes with CRISPR/Cas9
por: Fleiss, Aubin, et al.
Publicado: (2019)

CRISPR Cas9 in Pancreatic Cancer Research
por: Yang, Hai, et al.
Publicado: (2019)

CRISPR/Cas9 or prime editing? – It depends on…
por: Schenke, Dirk
Publicado: (2020)

Application of CRISPR/Cas9 in Alzheimer’s Disease
por: Lu, Likui, et al.
Publicado: (2021)

CRISPR/Cas9 therapeutics: progress and prospects
por: Li, Tianxiang, et al.
Publicado: (2023)

CRISPR-Cas9/Cas12a biotechnology and application in bacteria
por: Yao, Ruilian, et al.
Publicado: (2018)

Development and Applications of CRISPR/Cas9-Based Genome Editing in Lactobacillus
por: Mu, Yulin, et al.
Publicado: (2022)

Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife
por: Meaker, Grace A, et al.
Publicado: (2020)

Structural basis for the activation of a compact CRISPR-Cas13 nuclease
por: Deng, Xiangyu, et al.
Publicado: (2023)

Structural basis for inhibition of the type I-F CRISPR–Cas surveillance complex by AcrIF4, AcrIF7 and AcrIF14
por: Gabel, Clinton, et al.
Publicado: (2020)

DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes
por: Plagens, André, et al.
Publicado: (2015)

Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9
por: Zhong, Aaron, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_risbgufeieficfc6l5p7p75kfg