Cargando…
Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function
To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited. This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W,...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907229/ https://www.ncbi.nlm.nih.gov/pubmed/35264695 http://dx.doi.org/10.1038/s41598-022-08008-3 |
_version_ | 1784665593059737600 |
---|---|
author | Tongkaew, Patthamawadee Tohraman, Anna Bungaramphai, Ramlatee Mitrpant, Chalermchai Aydin, Ebru |
author_facet | Tongkaew, Patthamawadee Tohraman, Anna Bungaramphai, Ramlatee Mitrpant, Chalermchai Aydin, Ebru |
author_sort | Tongkaew, Patthamawadee |
collection | PubMed |
description | To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited. This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS. Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (−)-epicatechin and gallic acid) and the antidiabetic drug acarbose. (−)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD. Furthermore, unidentified polyphenol peaks of Kluai Hin were further explored by HPLC-ESI-QTOF-MS. The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g). A:W:A extraction of the fresh and freeze-dried peel of exhibited IC(50) values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC(50) = 0.25 ± 0.01 mg/ml). Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect. In addition, all standards, except gallocatechin, activated α-amylase activity, while, gallocatechin inhibited α-glucosidase activity better than acarbose. Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug. |
format | Online Article Text |
id | pubmed-8907229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89072292022-03-11 Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function Tongkaew, Patthamawadee Tohraman, Anna Bungaramphai, Ramlatee Mitrpant, Chalermchai Aydin, Ebru Sci Rep Article To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited. This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS. Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (−)-epicatechin and gallic acid) and the antidiabetic drug acarbose. (−)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD. Furthermore, unidentified polyphenol peaks of Kluai Hin were further explored by HPLC-ESI-QTOF-MS. The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g). A:W:A extraction of the fresh and freeze-dried peel of exhibited IC(50) values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC(50) = 0.25 ± 0.01 mg/ml). Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect. In addition, all standards, except gallocatechin, activated α-amylase activity, while, gallocatechin inhibited α-glucosidase activity better than acarbose. Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug. Nature Publishing Group UK 2022-03-09 /pmc/articles/PMC8907229/ /pubmed/35264695 http://dx.doi.org/10.1038/s41598-022-08008-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Tongkaew, Patthamawadee Tohraman, Anna Bungaramphai, Ramlatee Mitrpant, Chalermchai Aydin, Ebru Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function |
title | Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function |
title_full | Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function |
title_fullStr | Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function |
title_full_unstemmed | Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function |
title_short | Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function |
title_sort | kluai hin (musa sapientum linn.) peel as a source of functional polyphenols identified by hplc-esi-qtof-ms and its potential antidiabetic function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907229/ https://www.ncbi.nlm.nih.gov/pubmed/35264695 http://dx.doi.org/10.1038/s41598-022-08008-3 |
work_keys_str_mv | AT tongkaewpatthamawadee kluaihinmusasapientumlinnpeelasasourceoffunctionalpolyphenolsidentifiedbyhplcesiqtofmsanditspotentialantidiabeticfunction AT tohramananna kluaihinmusasapientumlinnpeelasasourceoffunctionalpolyphenolsidentifiedbyhplcesiqtofmsanditspotentialantidiabeticfunction AT bungaramphairamlatee kluaihinmusasapientumlinnpeelasasourceoffunctionalpolyphenolsidentifiedbyhplcesiqtofmsanditspotentialantidiabeticfunction AT mitrpantchalermchai kluaihinmusasapientumlinnpeelasasourceoffunctionalpolyphenolsidentifiedbyhplcesiqtofmsanditspotentialantidiabeticfunction AT aydinebru kluaihinmusasapientumlinnpeelasasourceoffunctionalpolyphenolsidentifiedbyhplcesiqtofmsanditspotentialantidiabeticfunction |