PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe

Mostrar otras versiones (1)

The in vivo-generator radionuclides (140)Nd (t(1/2) = 3.4 d) and (134)Ce (t(1/2) = 3.2 d) were used to trace a urokinase-type plasminogen activator (uPA)-targeting mouse monoclonal antibody, ATN-291, in U87 MG xenograft tumor-bearing mice. ATN-291 is known to internalize on the uPA/uPA-receptor pair...

Descripción completa

Detalles Bibliográficos
Autores principales: Severin, G. W., Fonslet, J., Kristensen, L. K., Nielsen, C. H., Jensen, A. I., Kjær, A., Mazar, A. P., Johnston, K., Köster, U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907281/
https://www.ncbi.nlm.nih.gov/pubmed/35264588
http://dx.doi.org/10.1038/s41598-022-07147-x
_version_ 1784665606179520512
author Severin, G. W.
Fonslet, J.
Kristensen, L. K.
Nielsen, C. H.
Jensen, A. I.
Kjær, A.
Mazar, A. P.
Johnston, K.
Köster, U.
author_facet Severin, G. W.
Fonslet, J.
Kristensen, L. K.
Nielsen, C. H.
Jensen, A. I.
Kjær, A.
Mazar, A. P.
Johnston, K.
Köster, U.
author_sort Severin, G. W.
collection PubMed
description The in vivo-generator radionuclides (140)Nd (t(1/2) = 3.4 d) and (134)Ce (t(1/2) = 3.2 d) were used to trace a urokinase-type plasminogen activator (uPA)-targeting mouse monoclonal antibody, ATN-291, in U87 MG xenograft tumor-bearing mice. ATN-291 is known to internalize on the uPA/uPA-receptor pair, making it an appropriate targeting vector for investigating the fate of in vivo generator daughters on internalizing probes. Ante-mortem and post-mortem PET imaging at 120 h post-injection gave no indication of redistribution of the positron emitting daughter nuclides (134)La and (140)Pr from tumor tissue (p > 0.5). The lack of redistribution indicates that the parent radionuclides (134)Ce and (140)Nd could be considered as long-lived PET-diagnostic matches to therapeutic radionuclides like (177)Lu, (161)Tb and (225)Ac when internalizing bioconjugates are employed.
format Online
Article
Text
id pubmed-8907281
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-89072812022-03-11 PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe Severin, G. W. Fonslet, J. Kristensen, L. K. Nielsen, C. H. Jensen, A. I. Kjær, A. Mazar, A. P. Johnston, K. Köster, U. Sci Rep Article The in vivo-generator radionuclides (140)Nd (t(1/2) = 3.4 d) and (134)Ce (t(1/2) = 3.2 d) were used to trace a urokinase-type plasminogen activator (uPA)-targeting mouse monoclonal antibody, ATN-291, in U87 MG xenograft tumor-bearing mice. ATN-291 is known to internalize on the uPA/uPA-receptor pair, making it an appropriate targeting vector for investigating the fate of in vivo generator daughters on internalizing probes. Ante-mortem and post-mortem PET imaging at 120 h post-injection gave no indication of redistribution of the positron emitting daughter nuclides (134)La and (140)Pr from tumor tissue (p > 0.5). The lack of redistribution indicates that the parent radionuclides (134)Ce and (140)Nd could be considered as long-lived PET-diagnostic matches to therapeutic radionuclides like (177)Lu, (161)Tb and (225)Ac when internalizing bioconjugates are employed. Nature Publishing Group UK 2022-03-09 /pmc/articles/PMC8907281/ /pubmed/35264588 http://dx.doi.org/10.1038/s41598-022-07147-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Severin, G. W.
Fonslet, J.
Kristensen, L. K.
Nielsen, C. H.
Jensen, A. I.
Kjær, A.
Mazar, A. P.
Johnston, K.
Köster, U.
PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe
title PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe
title_full PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe
title_fullStr PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe
title_full_unstemmed PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe
title_short PET in vivo generators (134)Ce and (140)Nd on an internalizing monoclonal antibody probe
title_sort pet in vivo generators (134)ce and (140)nd on an internalizing monoclonal antibody probe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907281/
https://www.ncbi.nlm.nih.gov/pubmed/35264588
http://dx.doi.org/10.1038/s41598-022-07147-x
work_keys_str_mv AT severingw petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT fonsletj petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT kristensenlk petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT nielsench petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT jensenai petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT kjæra petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT mazarap petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT johnstonk petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe
AT kosteru petinvivogenerators134ceand140ndonaninternalizingmonoclonalantibodyprobe

Ejemplares similares