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Genetic Dissection of CRISPR-Cas9 Mediated Inheritance of Independently Targeted Alleles in Tobacco α-1,3-Fucosyltransferase 1 and β-1,2-Xylosyltransferase 1 Loci

We determined the specificity of mutations induced by the CRISPR-Cas9 gene-editing system in tobacco (Nicotiana benthamiana) alleles and subsequent genetic stability. For this, we prepared 248 mutant plants using an Agrobacterium-delivered CRISPR-Cas9 system targeting α-1,3-fucosyltransferase 1 (Fuc...

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Detalles Bibliográficos
Autores principales: Song, Hayoung, Ahn, Ju-Young, Yan, Fanzhuang, Ran, Yidong, Koo, Okjae, Lee, Geung-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910323/
https://www.ncbi.nlm.nih.gov/pubmed/35269602
http://dx.doi.org/10.3390/ijms23052450