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Rapid detection of Salmonella in poultry environmental samples using real-time PCR coupled with immunomagnetic separation and whole genome amplification

We evaluated the combination of immunomagnetic separation (IMS), multiple displacement amplification (MDA), and real-time PCR to detect Salmonella from poultry environmental samples. The limits of detection (LODs) of IMS-MDA real-time PCR with different culture enrichment hours (0, 4, 6, and 8 h) we...

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Detalles Bibliográficos
Autores principales: Hyeon, Ji-Yeon, Mann, David A., Wang, Jinquan, Kim, Woo Kyun, Deng, Xiangyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913963/
https://www.ncbi.nlm.nih.gov/pubmed/31347691
http://dx.doi.org/10.3382/ps/pez425