A digital PCR-based protocol to detect and quantify RNA editing events at hotspots

APOBEC3A, CRISPR programmable RNA base editors, or other enzymes can edit RNA transcripts at specific locations or hotspots. Precise quantification of these RNA-editing events is crucial to determine the activity and efficiency of these enzymes in cells. We have developed a quick method to quantify...

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Detalles Bibliográficos
Autores principales: Oh, Sunwoo, Buisson, Rémi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915000/
https://www.ncbi.nlm.nih.gov/pubmed/35284835
http://dx.doi.org/10.1016/j.xpro.2022.101148

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915000/
https://www.ncbi.nlm.nih.gov/pubmed/35284835
http://dx.doi.org/10.1016/j.xpro.2022.101148

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