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A digital PCR-based protocol to detect and quantify RNA editing events at hotspots
APOBEC3A, CRISPR programmable RNA base editors, or other enzymes can edit RNA transcripts at specific locations or hotspots. Precise quantification of these RNA-editing events is crucial to determine the activity and efficiency of these enzymes in cells. We have developed a quick method to quantify...
Autores principales: | Oh, Sunwoo, Buisson, Rémi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915000/ https://www.ncbi.nlm.nih.gov/pubmed/35284835 http://dx.doi.org/10.1016/j.xpro.2022.101148 |
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