Cargando…
Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915010/ https://www.ncbi.nlm.nih.gov/pubmed/35284841 http://dx.doi.org/10.1016/j.xpro.2022.101230 |
_version_ | 1784667902110072832 |
---|---|
author | Dark, Callum Cheung, Shane Cheng, Louise Y. |
author_facet | Dark, Callum Cheung, Shane Cheng, Louise Y. |
author_sort | Dark, Callum |
collection | PubMed |
description | Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021). |
format | Online Article Text |
id | pubmed-8915010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89150102022-03-12 Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae Dark, Callum Cheung, Shane Cheng, Louise Y. STAR Protoc Protocol Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021). Elsevier 2022-03-09 /pmc/articles/PMC8915010/ /pubmed/35284841 http://dx.doi.org/10.1016/j.xpro.2022.101230 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Dark, Callum Cheung, Shane Cheng, Louise Y. Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae |
title | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae |
title_full | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae |
title_fullStr | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae |
title_full_unstemmed | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae |
title_short | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae |
title_sort | analyzing cachectic phenotypes in the muscle and fat body of drosophila larvae |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915010/ https://www.ncbi.nlm.nih.gov/pubmed/35284841 http://dx.doi.org/10.1016/j.xpro.2022.101230 |
work_keys_str_mv | AT darkcallum analyzingcachecticphenotypesinthemuscleandfatbodyofdrosophilalarvae AT cheungshane analyzingcachecticphenotypesinthemuscleandfatbodyofdrosophilalarvae AT chenglouisey analyzingcachecticphenotypesinthemuscleandfatbodyofdrosophilalarvae |