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Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae

Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications...

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Detalles Bibliográficos
Autores principales: Dark, Callum, Cheung, Shane, Cheng, Louise Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915010/
https://www.ncbi.nlm.nih.gov/pubmed/35284841
http://dx.doi.org/10.1016/j.xpro.2022.101230
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author Dark, Callum
Cheung, Shane
Cheng, Louise Y.
author_facet Dark, Callum
Cheung, Shane
Cheng, Louise Y.
author_sort Dark, Callum
collection PubMed
description Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021).
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spelling pubmed-89150102022-03-12 Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae Dark, Callum Cheung, Shane Cheng, Louise Y. STAR Protoc Protocol Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021). Elsevier 2022-03-09 /pmc/articles/PMC8915010/ /pubmed/35284841 http://dx.doi.org/10.1016/j.xpro.2022.101230 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Dark, Callum
Cheung, Shane
Cheng, Louise Y.
Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
title Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
title_full Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
title_fullStr Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
title_full_unstemmed Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
title_short Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae
title_sort analyzing cachectic phenotypes in the muscle and fat body of drosophila larvae
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915010/
https://www.ncbi.nlm.nih.gov/pubmed/35284841
http://dx.doi.org/10.1016/j.xpro.2022.101230
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