Cargando…

DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay

Dimethyl sulfoxide (DMSO) is a popular solvent for developmental toxicity testing of chemicals and pharmaceuticals in zebrafish embryos. In general, it is recommended to keep the final DMSO concentration as low as possible for zebrafish embryos, preferably not exceeding 100 μL/L (0.01%). However, hi...

Descripción completa

Detalles Bibliográficos
Autores principales: Hoyberghs, Jente, Bars, Chloé, Ayuso, Miriam, Van Ginneken, Chris, Foubert, Kenn, Van Cruchten, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915880/
https://www.ncbi.nlm.nih.gov/pubmed/35295145
http://dx.doi.org/10.3389/ftox.2021.804033
_version_ 1784668154352369664
author Hoyberghs, Jente
Bars, Chloé
Ayuso, Miriam
Van Ginneken, Chris
Foubert, Kenn
Van Cruchten, Steven
author_facet Hoyberghs, Jente
Bars, Chloé
Ayuso, Miriam
Van Ginneken, Chris
Foubert, Kenn
Van Cruchten, Steven
author_sort Hoyberghs, Jente
collection PubMed
description Dimethyl sulfoxide (DMSO) is a popular solvent for developmental toxicity testing of chemicals and pharmaceuticals in zebrafish embryos. In general, it is recommended to keep the final DMSO concentration as low as possible for zebrafish embryos, preferably not exceeding 100 μL/L (0.01%). However, higher concentrations of DMSO are often required to dissolve compounds in an aqueous medium. The aim of this study was to determine the highest concentration of DMSO that can be safely used in our standardized Zebrafish Embryo Developmental Toxicity Assay (ZEDTA). In the first part of this study, zebrafish embryos were exposed to different concentrations (0–2%) of DMSO. No increase in lethality or malformations was observed when using DMSO concentrations up to 1%. In a follow-up experiment, we assessed whether compounds that cause no developmental toxicity in the ZEDTA remain negative when dissolved in 1% DMSO, as false positive results due to physiological disturbances by DMSO should be avoided. To this end, zebrafish embryos were exposed to ascorbic acid and hydrochlorothiazide dissolved in 1% DMSO. Negative control groups were also included. No significant increase in malformations or lethality was observed in any of the groups. In conclusion, DMSO concentrations up to 1% can be safely used to dissolve compounds in the ZEDTA.
format Online
Article
Text
id pubmed-8915880
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89158802022-03-15 DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay Hoyberghs, Jente Bars, Chloé Ayuso, Miriam Van Ginneken, Chris Foubert, Kenn Van Cruchten, Steven Front Toxicol Toxicology Dimethyl sulfoxide (DMSO) is a popular solvent for developmental toxicity testing of chemicals and pharmaceuticals in zebrafish embryos. In general, it is recommended to keep the final DMSO concentration as low as possible for zebrafish embryos, preferably not exceeding 100 μL/L (0.01%). However, higher concentrations of DMSO are often required to dissolve compounds in an aqueous medium. The aim of this study was to determine the highest concentration of DMSO that can be safely used in our standardized Zebrafish Embryo Developmental Toxicity Assay (ZEDTA). In the first part of this study, zebrafish embryos were exposed to different concentrations (0–2%) of DMSO. No increase in lethality or malformations was observed when using DMSO concentrations up to 1%. In a follow-up experiment, we assessed whether compounds that cause no developmental toxicity in the ZEDTA remain negative when dissolved in 1% DMSO, as false positive results due to physiological disturbances by DMSO should be avoided. To this end, zebrafish embryos were exposed to ascorbic acid and hydrochlorothiazide dissolved in 1% DMSO. Negative control groups were also included. No significant increase in malformations or lethality was observed in any of the groups. In conclusion, DMSO concentrations up to 1% can be safely used to dissolve compounds in the ZEDTA. Frontiers Media S.A. 2021-12-21 /pmc/articles/PMC8915880/ /pubmed/35295145 http://dx.doi.org/10.3389/ftox.2021.804033 Text en Copyright © 2021 Hoyberghs, Bars, Ayuso, Van Ginneken, Foubert and Van Cruchten. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Toxicology
Hoyberghs, Jente
Bars, Chloé
Ayuso, Miriam
Van Ginneken, Chris
Foubert, Kenn
Van Cruchten, Steven
DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay
title DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay
title_full DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay
title_fullStr DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay
title_full_unstemmed DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay
title_short DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay
title_sort dmso concentrations up to 1% are safe to be used in the zebrafish embryo developmental toxicity assay
topic Toxicology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915880/
https://www.ncbi.nlm.nih.gov/pubmed/35295145
http://dx.doi.org/10.3389/ftox.2021.804033
work_keys_str_mv AT hoyberghsjente dmsoconcentrationsupto1aresafetobeusedinthezebrafishembryodevelopmentaltoxicityassay
AT barschloe dmsoconcentrationsupto1aresafetobeusedinthezebrafishembryodevelopmentaltoxicityassay
AT ayusomiriam dmsoconcentrationsupto1aresafetobeusedinthezebrafishembryodevelopmentaltoxicityassay
AT vanginnekenchris dmsoconcentrationsupto1aresafetobeusedinthezebrafishembryodevelopmentaltoxicityassay
AT foubertkenn dmsoconcentrationsupto1aresafetobeusedinthezebrafishembryodevelopmentaltoxicityassay
AT vancruchtensteven dmsoconcentrationsupto1aresafetobeusedinthezebrafishembryodevelopmentaltoxicityassay