A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers

BACKGROUND: Site-directed mutagenesis (SDM) is a key method in molecular biology; allowing to modify DNA sequences at single base pair resolution. Although many SDM methods have been developed, methods that increase efficiency and versatility of this process remain highly desired. METHOD: We present...

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Detalles Bibliográficos
Autores principales: Olszakier, Shunit, Berlin, Shai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918331/
https://www.ncbi.nlm.nih.gov/pubmed/35282829
http://dx.doi.org/10.1186/s12896-022-00740-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918331/
https://www.ncbi.nlm.nih.gov/pubmed/35282829
http://dx.doi.org/10.1186/s12896-022-00740-y

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