Protocol for isolation and functional validation of label-retaining quiescent colorectal cancer stem cells from patient-derived organoids for RNA-seq

Quiescent cancer stem cells (qCSCs) are a major source of posttreatment relapse, but methods to identify molecular targets for qCSC elimination are limited. Here, we present a protocol using the fluorescent dye PKH26 to isolate label-retaining qCSCs from colorectal cancer (CRC) patient-derived organ...

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Detalles Bibliográficos
Autor principal: Regan, Joseph L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920924/
https://www.ncbi.nlm.nih.gov/pubmed/35300001
http://dx.doi.org/10.1016/j.xpro.2022.101225

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920924/
https://www.ncbi.nlm.nih.gov/pubmed/35300001
http://dx.doi.org/10.1016/j.xpro.2022.101225

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