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Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9

The zebra finch is one of the most commonly studied songbirds in biology, particularly in genomics, neuroscience and vocal communication. However, this species lacks a robust cell line for molecular biology research and reagent optimization. We generated a cell line, designated CFS414, from zebra fi...

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Detalles Bibliográficos
Autores principales: Biegler, Matthew T., Fedrigo, Olivier, Collier, Paul, Mountcastle, Jacquelyn, Haase, Bettina, Tilgner, Hagen U., Jarvis, Erich D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921232/
https://www.ncbi.nlm.nih.gov/pubmed/35288582
http://dx.doi.org/10.1038/s41598-022-07434-7