A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity

The S. pyogenes (Sp) Cas9 endonuclease is an important gene-editing tool. SpCas9 is directed to target sites based on complementarity to a complexed single-guide RNA (sgRNA). However, SpCas9-sgRNA also binds and cleaves genomic off-targets with only partial complementarity. To date, we lack the abil...

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Detalles Bibliográficos
Autores principales: Eslami-Mossallam, Behrouz, Klein, Misha, Smagt, Constantijn V. D., Sanden, Koen V. D., Jones, Stephen K., Hawkins, John A., Finkelstein, Ilya J., Depken, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924176/
https://www.ncbi.nlm.nih.gov/pubmed/35292641
http://dx.doi.org/10.1038/s41467-022-28994-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924176/
https://www.ncbi.nlm.nih.gov/pubmed/35292641
http://dx.doi.org/10.1038/s41467-022-28994-2

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