Cargando…
A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity
The S. pyogenes (Sp) Cas9 endonuclease is an important gene-editing tool. SpCas9 is directed to target sites based on complementarity to a complexed single-guide RNA (sgRNA). However, SpCas9-sgRNA also binds and cleaves genomic off-targets with only partial complementarity. To date, we lack the abil...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924176/ https://www.ncbi.nlm.nih.gov/pubmed/35292641 http://dx.doi.org/10.1038/s41467-022-28994-2 |