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Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter

BACKGROUND: Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes. Ribozyme-mediated CRISPR/Cas9 systems increase the types...

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Detalles Bibliográficos
Autores principales: Li, Jia-Wen, Zeng, Tuo, Xu, Zhi-Zhuo, Li, Jin-Jin, Hu, Hao, Yu, Qin, Zhou, Li, Zheng, Ri-Ru, Luo, Jing, Wang, Cai-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925089/
https://www.ncbi.nlm.nih.gov/pubmed/35292048
http://dx.doi.org/10.1186/s13007-022-00863-5