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Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter
BACKGROUND: Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes. Ribozyme-mediated CRISPR/Cas9 systems increase the types...
Autores principales: | Li, Jia-Wen, Zeng, Tuo, Xu, Zhi-Zhuo, Li, Jin-Jin, Hu, Hao, Yu, Qin, Zhou, Li, Zheng, Ri-Ru, Luo, Jing, Wang, Cai-Yun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925089/ https://www.ncbi.nlm.nih.gov/pubmed/35292048 http://dx.doi.org/10.1186/s13007-022-00863-5 |
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