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A decontamination strategy for resolving SARS-CoV-2 amplicon contamination in a next-generation sequencing laboratory

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) amplicon contamination was discovered due to next-generation sequencing (NGS) reads mapping in the negative controls. Environmental screening was undertaken to determine the source of contamination, which was suspected to be evaporati...

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Detalles Bibliográficos
Autores principales: Mwangi, Peter, Mogotsi, Milton, Ogunbayo, Ayodeji, Mooko, Teboho, Maringa, Wairimu, Sondlane, Hlengiwe, Nkwadipo, Kelebogile, Adelabu, Olusesan, Bester, Philip Armand, Goedhals, Dominique, Nyaga, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926888/
https://www.ncbi.nlm.nih.gov/pubmed/35298714
http://dx.doi.org/10.1007/s00705-022-05411-z