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CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase II (Pol2...

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Detalles Bibliográficos
Autores principales: Janssens, Derek H., Otto, Dominik J., Meers, Michael P., Setty, Manu, Ahmad, Kami, Henikoff, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928696/
https://www.ncbi.nlm.nih.gov/pubmed/35300717
http://dx.doi.org/10.1186/s13059-022-02642-w