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Effects of operational taxonomic unit inference methods on soil microeukaryote community analysis using long‐read metabarcoding

Long amplicon metabarcoding has opened the door for phylogenetic analysis of the largely unknown communities of microeukaryotes in soil. Here, we amplified and sequenced the ITS and LSU regions of the rDNA operon (around 1500 bp) from grassland soils using PacBio SMRT sequencing. We tested how three...

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Detalles Bibliográficos
Autores principales: Eshghi Sahraei, Shadi, Furneaux, Brendan, Kluting, Kerri, Zakieh, Mustafa, Rydin, Håkan, Hytteborn, Håkan, Rosling, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928899/
https://www.ncbi.nlm.nih.gov/pubmed/35342585
http://dx.doi.org/10.1002/ece3.8676