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The Small Metal-Binding Protein SmbP Improves the Expression and Purification of the Recombinant Antitumor-Analgesic Peptide from the Chinese Scorpion Buthus martensii Karsch in Escherichia coli

We have recently shown that SmbP, the small metal-binding protein of Nitrosomonas europaea, can be employed as a fusion protein to express and purify recombinant proteins and peptides in Escherichia coli. SmbP increases solubility, allows simple, one-step purification through affinity chromatography...

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Detalles Bibliográficos
Autores principales: Martinez-Mora, Evelyn, Arredondo-Espinoza, Eder, Casillas-Vega, Nestor G., Cantu-Cardenas, Maria Elena, Balderas-Renteria, Isaias, Zarate, Xristo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929023/
https://www.ncbi.nlm.nih.gov/pubmed/35723324
http://dx.doi.org/10.3390/cimb44020038