Determining the effects of loss of function mutations in human cell lines

Quantifying differences in the amount of protein and mRNA caused by missense mutations in a gene of interest can be challenging, especially when using patient-derived primary cells, which are intrinsically variable. In this protocol, we describe how to culture patient-derived lymphoblast and fibrobl...

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Detalles Bibliográficos
Autores principales: de Prisco, Nicola, Botta, Salvatore, Lee, Winston, Rezazadeh, Sarallah, Chemiakine, Alexei, Gennarino, Vincenzo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931413/
https://www.ncbi.nlm.nih.gov/pubmed/35310075
http://dx.doi.org/10.1016/j.xpro.2022.101232

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931413/
https://www.ncbi.nlm.nih.gov/pubmed/35310075
http://dx.doi.org/10.1016/j.xpro.2022.101232

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