Medium throughput protocol for genome-based quantification of intracellular mycobacterial loads and macrophage survival during in vitro infection

Here, we present a streamlined protocol for assessing intracellular Mycobacterium tuberculosis (Mtb) loads in macrophages. This protocol describes the simultaneous assessment of macrophage viability using automated microscopy. Further, we detail the quantification of mycobacterial loads using a rapi...

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Detalles Bibliográficos
Autores principales: Yabaji, Shivraj M., Chatterjee, Sujoy, Waligursky, Emily, Gimelbrant, Alexander, Kramnik, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931439/
https://www.ncbi.nlm.nih.gov/pubmed/35310069
http://dx.doi.org/10.1016/j.xpro.2022.101241

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931439/
https://www.ncbi.nlm.nih.gov/pubmed/35310069
http://dx.doi.org/10.1016/j.xpro.2022.101241

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