Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons

Genetic variants that affect neurological function will often produce changes visible at the level of gross morphology, either of the whole brain or of specific neuronal types. Here we describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we outline steps for culturin...

Descripción completa

Detalles Bibliográficos
Autores principales: de Prisco, Nicola, Chemiakine, Alexei, Lee, Winston, Botta, Salvatore, Gennarino, Vincenzo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931472/
https://www.ncbi.nlm.nih.gov/pubmed/35310074
http://dx.doi.org/10.1016/j.xpro.2022.101244
_version_ 1784671272491286528
author de Prisco, Nicola
Chemiakine, Alexei
Lee, Winston
Botta, Salvatore
Gennarino, Vincenzo A.
author_facet de Prisco, Nicola
Chemiakine, Alexei
Lee, Winston
Botta, Salvatore
Gennarino, Vincenzo A.
author_sort de Prisco, Nicola
collection PubMed
description Genetic variants that affect neurological function will often produce changes visible at the level of gross morphology, either of the whole brain or of specific neuronal types. Here we describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we outline steps for culturing mouse primary hippocampal neurons to evaluate dendritic arborization (Sholl analysis). For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018).
format Online
Article
Text
id pubmed-8931472
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-89314722022-03-19 Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons de Prisco, Nicola Chemiakine, Alexei Lee, Winston Botta, Salvatore Gennarino, Vincenzo A. STAR Protoc Protocol Genetic variants that affect neurological function will often produce changes visible at the level of gross morphology, either of the whole brain or of specific neuronal types. Here we describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we outline steps for culturing mouse primary hippocampal neurons to evaluate dendritic arborization (Sholl analysis). For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018). Elsevier 2022-03-15 /pmc/articles/PMC8931472/ /pubmed/35310074 http://dx.doi.org/10.1016/j.xpro.2022.101244 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
de Prisco, Nicola
Chemiakine, Alexei
Lee, Winston
Botta, Salvatore
Gennarino, Vincenzo A.
Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
title Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
title_full Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
title_fullStr Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
title_full_unstemmed Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
title_short Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
title_sort protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931472/
https://www.ncbi.nlm.nih.gov/pubmed/35310074
http://dx.doi.org/10.1016/j.xpro.2022.101244
work_keys_str_mv AT deprisconicola protocoltoassesstheeffectofdiseasedrivingvariantsonmousebrainmorphologyandprimaryhippocampalneurons
AT chemiakinealexei protocoltoassesstheeffectofdiseasedrivingvariantsonmousebrainmorphologyandprimaryhippocampalneurons
AT leewinston protocoltoassesstheeffectofdiseasedrivingvariantsonmousebrainmorphologyandprimaryhippocampalneurons
AT bottasalvatore protocoltoassesstheeffectofdiseasedrivingvariantsonmousebrainmorphologyandprimaryhippocampalneurons
AT gennarinovincenzoa protocoltoassesstheeffectofdiseasedrivingvariantsonmousebrainmorphologyandprimaryhippocampalneurons

Ejemplares similares